Synergistic Ability of Chitosan and Trichoderma harzianum to Control the Growth and Discolouration of Common Sapstain Fungi of Pinus radiata

An environmentally compatible method for controlling sapstain fungi in wood was evaluated, using a combination of chitosan and an albino strain Trichoderma harzianum, biological control agent (BCA). The growth penetration into the Ophiostoma piceae, Leptographium procerum, Sphaeropsis sapinea were assessed radiata pine wafers treated with BCA, both alone combination. Several mycological microscopic techniques used, including gfp (green fluorescent protein) transformed O. piceae assessing depth samples. synergy between BCA evident, two tested fungi, only afforded protection. synnemata (recognized by erect conidiogenous cells bearing conidia) observed on surface inoculated L. procerum but hyphae unable to penetrate melanise. results suggest that limited ability deeply compensated fast T. harzianum inner wood.